Abstract: Immunomodulation in Vascularized Composite Allotransplantation – Preliminary Results in a Non-human Primate Model with Tocilizumab

نویسندگان

  • Zhi Yang Ng
  • Alexandre G. Lellouch
  • Matthew W. Defazio
  • Zachary W. Heroux
  • Jigesh A. Shah
  • Josef M. Kurtz
  • Curtis L. Cetrulo
چکیده

MATERIALS AND METHODS: 8 cynomolgus monkeys received either an orthotopic hand (n=2) or heterotopic face VCA (n=6) from MHC-mismatched donors following induction with anti-thymocyte globulin. Postoperatively, triple immunosuppression – tacrolimus, mycophenolate mofetil, methylprednisolone – was maintained for up to 120 days before bone marrow transplantation (BMT) was performed. Protocol biopsies of VCA skin were performed at 30-day intervals for histopathology and flow cytometric analysis of resident skin leukocyte populations; VCA-resident cells were differentiated by H38 status (mouse antihuman HLA class I monoclonal antibody that cross reacts with cynomolgus monkeys) for donor or recipient derivation. Clinical AR was treated with steroids and further biopsies were taken for histologic confirmation; corresponding anti-donor responses were evaluated by mixed lymphocyte reaction (MLR) and allo-antibody formation.

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عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2016